Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.

نویسندگان

  • C A Helliwell
  • A Poole
  • W J Peacock
  • E S Dennis
چکیده

The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.

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عنوان ژورنال:
  • Plant physiology

دوره 119 2  شماره 

صفحات  -

تاریخ انتشار 1999